Linking Epitope-Specific T-Cell Receptors to IFNγ Secretion Using Nanovial Technology
Jet van den Dijssel, Tejas Menon, Hayley A. McQuilten, Oliver Eltherington, Alexis Gonzalez, Sevana Baghdasarian, Citradewi Soemardy, Dino Di Carlo, Klaas P.J.M. van Gisbergen, Anja ten Brinke, Carolien E. van de Sandt
European Journal of Immunology, May 2025
Abstract
To link IFNγ-secretion levels of epitope-specific T-cells with their TCRαβ, we coated Nanovials with pHLA-I to capture and activate epitope-specific T-cells and their secreted IFNγ, followed by index-sorting and TCRαβ sequencing. We demonstrate that Nanovials are a promising tool to link single epitope-specific TCRαβ clonotypes to the cell's functional properties.
Topics
Cytokine Profiling
Cell Types
Human T cells
Secretion Targets
IFNγ, TNF-alpha